Multicomponent content determination and quality analysis of Mume Flos at different flowering stages based on UPLC

In order to compare the quality differences of Mume Flos at different flowering stages, 45 batches of Mume Flos at different growth and flowering stages were collected. UPLC method was used to establish a method for simultaneous determination of nine components in Mume Flos. On this basis, combined with stoichiometric analysis, 45 batches of Mume Flos were identified and classified by cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). The results showed that the established method could achieve good separation of each index component in the sample, and the content of phenolic acids such as neochlorogenic acid and chlorogenic acid decreases with the increase of growth period. The contents of flavonoids such as hypericin, isoquercetin, rutin, quercetin-3-O-neohesperidin were higher in the full flowering period than in other …