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To establish the fingerprints of Euodiae Fructus and prepared Euodiae Fructus, the changes of chemical components in Euodiae Fructus and its preparation were analyzed by multivariate statistics. Using ultra high performance liquid chromatography (UPLC) technology. Chromatographic column was YMC Triart C 18 (100 mm× 2.1 mm, 1.9 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid water (gradient elution), the flow rate was 0.30 mL/min, the column temperature was 30℃, the detection wavelength was 254 nm, the injection volume was 1 μL. The UPLC fingerprints of Euodiae Fructus and its preparation were established, and the similarity calculation and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze and evaluate the fingerprints, the variable importance projection (VIP) value greater than 1 was used as the criterion to screen the differential …